abstract：:Contradictory results for concentrations of vitamin B12, holotranscobalamin (holoTC), and methylmalonic acid (MMA) have been reported. We tested the hypothesis that the extracellular vitamin B12 markers are not reflecting the intracellular vitamin B12-dependent biochemical reactions in individuals with type 2 diabetes...

journal_title：Biochimie

authors： Obeid R,Jung J,Falk J,Herrmann W,Geisel J,Friesenhahn-Ochs B,Lammert F,Fassbender K,Kostopoulos P

更新日期：2013-05-01 00:00:00

abstract：:A DNA sequence encoding a protein with predicted EF-hand and dynein light chain binding domains was identified in a Fasciola hepatica EST library. Sequence analysis of the encoded protein revealed that the most similar known protein was the Fasciola gigantica protein FgCaBP3 and so this newly identified protein was na...

journal_title：Biochimie

authors： Banford S,Drysdale O,Hoey EM,Trudgett A,Timson DJ

更新日期：2013-04-01 00:00:00

abstract：:High-mobility group box-1 (HMGB1) is remarkably mobile in living cells, which reflects its ability to interact only transiently with both DNA and protein. This property is likely essential for HMGB1 nuclear activities. Nonetheless the weak interaction of HMGB1 with DNA and/or protein partners has also been a major lim...

journal_title：Biochimie

authors： Messmer M,Klein C,Boniface R,Gnädig NF,Lecerf M,Barnay-Verdier S,Maréchal V

更新日期：2013-04-01 00:00:00

abstract：:The concept of a stem cell subpopulation as understood from normal epithelial tissue or bone marrow function has been extended to our understanding of cancer tissue and is now the target of treatment efforts specifically directed to this subpopulation. In glioblastoma, as well as in other cancers, increased expression...

journal_title：Biochimie

authors： Rappa F,Cappello F,Halatsch ME,Scheuerle A,Kast RE

更新日期：2013-04-01 00:00:00

abstract：:For efficient DNA hydrolysis, Type III restriction endonuclease EcoP15I interacts with two inversely oriented recognition sites in an ATP-dependent process. EcoP15I consists of two methylation (Mod) subunits and a single restriction (Res) subunit yielding a multifunctional enzyme complex able to methylate or to hydrol...

journal_title：Biochimie

authors： Mackeldanz P,Alves J,Möncke-Buchner E,Wyszomirski KH,Krüger DH,Reuter M

更新日期：2013-04-01 00:00:00

abstract：:Low bone formation in osteoporosis is associated with a shift from osteoblastic to adipogenic differentiation of mesenchymal stem cells (MSC) inducing a concomitant lipotoxic milieu within the bone marrow. Strontium ranelate (SrRN), a treatment for osteoporosis, has both anti-resorptive and anabolic effects on bone. T...

journal_title：Biochimie

authors： Vidal C,Gunaratnam K,Tong J,Duque G

更新日期：2013-04-01 00:00:00

abstract：:Protein l-isoaspartyl-O-methyltransferase (PIMT) is an ubiquitous enzyme widely distributed in cells and plays a role in the repair of deamidated and isomerized proteins. In this study, we show that this enzyme is present in cytosolic extract of Vibrio cholerae, an enteric pathogenic Gram-negative bacterium and is enz...

journal_title：Biochimie

authors： Chatterjee T,Pal A,Chakravarty D,Dey S,Saha RP,Chakrabarti P

更新日期：2013-04-01 00:00:00

abstract：:I have been involved in research on oxysterols since 1963 and this review is intended to cover some of the most important aspects of this work. The first project dealed with 7α-hydroxy-4-cholesten-3-one. My successful synthesis of this steroid with high specific radioactivity allowed a demonstration that it is a bile ...

journal_title：Biochimie

authors： Björkhem I

更新日期：2013-03-01 00:00:00

abstract：:Many human conditions, including neurological diseases, atherosclerosis, cancer, diabetic complications and aging, are thought to be associated with oxidative stress (OS). The development of reliable and informative markers for the characterization of OS in humans is thus highly important. Various endogenous markers a...

journal_title：Biochimie

authors： Vaya J

更新日期：2013-03-01 00:00:00

abstract：:Several mechanistic hypotheses have been proposed for how carotenoid pigmentation of integumentary structures can serve as an honest signal of individual quality. These hypotheses are founded on proposed links between carotenoids, immuno responsiveness, and oxidative stress, but an absence of biochemical information o...

journal_title：Biochimie

authors： Johnson JD,Hill GE

更新日期：2013-02-01 00:00:00

abstract：:SR-PSOX can function as a scavenger receptor, a chemokine and an adhesion molecule, and it could be an interesting player in the formation of atherosclerotic lesions. Our previous studies demonstrated that basic amino acid residues in the chemokine domain of SR-PSOX are critical for its functions. In this study the co...

journal_title：Biochimie

authors： Liu W,Yin L,Dai Y

更新日期：2013-02-01 00:00:00

abstract：:Secondary structure-forming DNA motifs have achieved infamy because of their association with a variety of human diseases and cancer. The 3rd FASEB summer conference on dynamic DNA structures focused on the mechanisms responsible for the instabilities inherent to repetitive DNA and presented many exciting and novel as...

journal_title：Biochimie

authors： Saini N,Zhang Y,Usdin K,Lobachev KS

更新日期：2013-02-01 00:00:00

abstract：:Human α(1)-acid glycoprotein (AGP) is a positive acute phase plasma protein containing two disulfide bridges. Structural studies have shown that under specific conditions AGP undergoes aggregation. In this study, we analysed the nature of AGP's aggregates formed under reducing and non-reducing conditions at pH 5.5 and...

journal_title：Biochimie

authors： Scirè A,Baldassarre M,Galeazzi R,Tanfani F

更新日期：2013-02-01 00:00:00

abstract：:Mitochondrial membranes are essential for the good functioning of the organelle. For instance, the inner mitochondrial membrane contains the oxidative phosphorylation system that permits ATP synthesis. Phospholipids environment and especially cardiolipin are crucial for the mitochondrial energy metabolism. Indeed, car...

journal_title：Biochimie

authors： Dumas JF,Peyta L,Couet C,Servais S

更新日期：2013-01-01 00:00:00

abstract：:The toxicity of amyloids is a subject under intense scrutiny. Many studies link this toxicity to the existence of various intermediate structures prior to the fiber formation and/or their specific interaction with membranes. Membranes can also be a catalyst of amyloidogenesis and the composition or the charge of membr...

journal_title：Biochimie

authors： Berthelot K,Cullin C,Lecomte S

更新日期：2013-01-01 00:00:00

abstract：:Lipidomics is a subspecialty of metabolomics that focuses on water insoluble metabolites that form membrane barriers. Most lipidomic databases catalog lipids from common model organisms, like humans or Escherichia coli. However, model organisms' lipid profiles show surprisingly little overlap with those of specialized...

journal_title：Biochimie

authors： Layre E,Moody DB

更新日期：2013-01-01 00:00:00

abstract：:Innate immune system is the first line of host defense against invading microorganisms. It relies on a limited number of germline-encoded pattern recognition receptors that recognize conserved molecular structures of microbes, referred to as pathogen-/microbe-associated molecular patterns (PAMPs/MAMPs). Bacterial cell...

journal_title：Biochimie

authors： Ray A,Cot M,Puzo G,Gilleron M,Nigou J

更新日期：2013-01-01 00:00:00

abstract：:Excessive tissue iron levels are associated with the increase of oxidative/nitrative stress which contributes to tissue damage that may elevate the risk of diabetes. Therefore, we investigated the effects of iron on diabetes-associated liver injury and whether iron-related tyrosine nitration participated in this proce...

journal_title：Biochimie

authors： Li X,Li H,Lu N,Feng Y,Huang Y,Gao Z

更新日期：2012-12-01 00:00:00

abstract：:The imprinted gene Grb10 influences fetal growth, adult behavior and tumor formation. Investigating this gene often involves consecutive passaging of cells in vitro. We analyzed changes in Grb10 DNA methylation and histone modifications in serially passaged adult mouse tail-tip fibroblast cells with bisulfite genomic ...

journal_title：Biochimie

authors： Yao J,Huang Y,Huang R,Shi R,Chen P,Zhu B,Li M,Jiang X,Zheng M,Jiang Y,Yang X

更新日期：2012-12-01 00:00:00

abstract：:Staphylococcus aureus is one of the most dreaded pathogens worldwide and emergence of notorious antibiotic resistant strains have further exacerbated the present scenario. The glycolytic enzyme, triosephosphate isomerase (TIM) is one of the cell envelope proteins of the coccus and is involved in biofilm formation. It ...

journal_title：Biochimie

authors： Mukherjee S,Roychowdhury A,Dutta D,Das AK

更新日期：2012-12-01 00:00:00

abstract：:Accumulating evidences suggest that Herba epimedii has the potential benefits against osteoporosis. However, previous studies were focused on the crude extract, total flavonoids (TF) and icariin (ICA), and the detailed molecular mechanisms of action and structure-activity relationship (SAR) remain unclear. Herein we a...

journal_title：Biochimie

authors： Zhang D,Zhang J,Fong C,Yao X,Yang M

更新日期：2012-12-01 00:00:00

abstract：:In this contribution, we have studied the dynamics of electron transfer (ET) of a flavoprotein to the bound cofactor, an important metabolic process, in a model molecular/macromolecular crowding environments. Vitamin B(2) (riboflavin, Rf) and riboflavin binding protein (RBP) are used as model cofactor and flavoprotein...

journal_title：Biochimie

authors： Rakshit S,Saha R,Verma PK,Mitra RK,Pal SK

更新日期：2012-12-01 00:00:00

abstract：:An 18.2 kDa protein from the liver fluke, Fasciola hepatica has been identified and characterised. The protein shows strongest sequence similarity to egg antigen proteins from Schistosoma mansoni, Schistosoma japonicum and Clonorchis sinensis. The protein is predicted to adopt a calmodulin-like fold; it thus represent...

journal_title：Biochimie

authors： Russell SL,McFerran NV,Moore CM,Tsang Y,Glass P,Hoey EM,Trudgett A,Timson DJ

更新日期：2012-11-01 00:00:00

abstract：:Fluorometry using a substrate DNA labeled with a single fluorophore (6-carboxyfluorescein) at the 3'-end of the processed strand was shown to be a useful tool for monitoring DNA-binding and 3'-processing activities of HIV-1 and PFV integrases (INs). The DNA binding to either of the INs resulted in a fluorescence signa...

journal_title：Biochimie

authors： Anisenko A,Agapkina J,Zatsepin T,Yanvarev D,Gottikh M

更新日期：2012-11-01 00:00:00

abstract：:Epigenetic mechanisms are likely to play a role in many complex diseases, the extent of which we only beginning to understand. COPD and asthma are two respiratory diseases subject to strong environmental influences depending on underlying genetic susceptibility. Epigenetic mechanisms such as DNA methylation, histone m...

journal_title：Biochimie

authors： Kabesch M,Adcock IM

更新日期：2012-11-01 00:00:00

abstract：:Epigenetic alterations are involved in every step of carcinogenesis. The development of chromatin-modifying agents (CMAs) has provided the ability to fight cancer by reversing these alterations. Currently, four CMAs have been approved for cancer treatment; two DNA demethylating agents and two deacetylase inhibitors. A...

journal_title：Biochimie

authors： Seidel C,Florean C,Schnekenburger M,Dicato M,Diederich M

更新日期：2012-11-01 00:00:00

abstract：:Adipose tissue is an endocrine organ that plays an essential role in regulating several metabolic functions through the secretion of biological mediators called "adipokines". Dysregulation of adipokines plays a crucial role in obesity-related diseases. Adiponectin (APN) is the most abundant adipokine accounting for th...

journal_title：Biochimie

authors： Villarreal-Molina MT,Antuna-Puente B

更新日期：2012-10-01 00:00:00

abstract：:Since the initial identification of leptin as the product of the ob gene in 1994, the signaling pathways by which this hormone alters cell physiology have been the subject of extensive investigations. The fact that leptin can induce nitric oxide (NO) production was first demonstrated in studies of the pituitary gland ...

journal_title：Biochimie

authors： Joffin N,Niang F,Forest C,Jaubert AM

更新日期：2012-10-01 00:00:00

abstract：:Our previous researches showed that nitronyl nitroxyl derivatives, NNP and NNVP were good anti-oxidants and provided radioprotective effects in C6 cells. The objective of the present study is to investigate the possible antiviral effects and underlying pharmacological of the two nitronyl nitroxide radicals against CVB...

journal_title：Biochimie

authors： Wang H,Gao P,Jing L,Qin X,Sun X

更新日期：2012-09-01 00:00:00

abstract：:Lysophosphatidic acid (LPA), a naturally occurring bioactive phospholipid, mediates a multitude of (patho)physiological events including activation of mitogen-activated protein kinases (MAPKs). As LPA may induce cellular reponses in human osteosarcoma, the present study aimed at investigating expression of various LPA...

journal_title：Biochimie

authors： Windischhofer W,Huber E,Rossmann C,Semlitsch M,Kitz K,Rauh A,Devaney T,Leis HJ,Malle E

更新日期：2012-09-01 00:00:00

abstract：:Dihydroorotate dehydrogenase (DHODH) is the fourth enzyme in the de novo pyrimidine biosynthetic pathway and has been exploited as the target for therapy against proliferative and parasitic diseases. In this study, we report the crystal structures of DHODH from Leishmania major, the species of Leishmania associated wi...

journal_title：Biochimie

authors： Cordeiro AT,Feliciano PR,Pinheiro MP,Nonato MC

更新日期：2012-08-01 00:00:00

abstract：:Vascular endothelial growth factor (VEGF) and αvβ3 integrin are key molecules that actively participate in tumor angiogenesis and metastasis. Some integrin-blocking molecules are currently under clinical trials for cancer and metastasis treatment. However, the mechanism of action of such inhibitors is not completely u...

journal_title：Biochimie

authors： Montenegro CF,Salla-Pontes CL,Ribeiro JU,Machado AZ,Ramos RF,Figueiredo CC,Morandi V,Selistre-de-Araujo HS

更新日期：2012-08-01 00:00:00

abstract：:Nucleic acid Aptamers are ligands that are selected by a process of molecular evolution to bind with high affinities and specificities to a specific target. Recently, an increasing number of aptamers have been selected against biomarkers expressed at the surface of human cells or infectious pathogens. This class of ta...

journal_title：Biochimie

authors： Cibiel A,Pestourie C,Ducongé F

更新日期：2012-07-01 00:00:00

abstract：:A large part of higher eukaryotic genomes is transcribed into RNAs lacking any significant open reading frame. This "non-coding part" has been shown to actively contribute to regulating gene expression, but the mechanisms are largely unknown. Particularly instructive examples are provided by the dosage compensation sy...

journal_title：Biochimie

authors： Maenner S,Müller M,Becker PB

更新日期：2012-07-01 00:00:00

abstract：:A non-toxic, direct-acting fibrinolytic serine protease (Bafibrinase) demonstrating thrombolytic and anticoagulant properties was purified from Bacillus sp. strain AS-S20-I. Bafibrinase was monomeric, with a molecular mass of 32.3 kDa. The peptide mass fingerprinting of Bafibrinase revealed only 8.3% sequence coverage...

journal_title：Biochimie

authors： Mukherjee AK,Rai SK,Thakur R,Chattopadhyay P,Kar SK

更新日期：2012-06-01 00:00:00

abstract：:In vertebrates, mannose 6-phosphate receptors [MPR300 (Mr 300 kDa) and MPR46 (Mr 46 kDa)] are highly conserved transmembrane glycoproteins that mediate transport of lysosomal enzymes to lysosomes. Our studies have revealed the appearance of these putative receptors in invertebrates such as the molluscs and deuterostom...

journal_title：Biochimie

authors： Visa M,Hammer E,Völker U,Koliwer-Brandl H,Kelm S,Nadimpalli SK

更新日期：2012-05-01 00:00:00

abstract：:We have recently reported that α(2)β(1) and α(1)β(1) isozymes of Na(+)/K(+)-ATPase (NKA) are localized in the caveolae whereas only the α(1)β(1) isozyme of NKA is localized in the non-caveolae fraction of pulmonary artery smooth muscle cell membrane. It is well known that different isoforms of NKA are regulated differ...

journal_title：Biochimie

authors： Dey K,Roy S,Ghosh B,Chakraborti S

更新日期：2012-04-01 00:00:00

abstract：:Glucose-6 phosphatase (G6Pase), a key enzyme of glucose homeostasis, catalyses the hydrolysis of glucose-6 phosphate (G6P) to glucose and inorganic phosphate. A deficiency in G6Pase activity causes type 1 glycogen storage disease (GSD-1), mainly characterised by hypoglycaemia. Genetic analyses of the two forms of this...

journal_title：Biochimie

authors： Soty M,Chilloux J,Casteras S,Grichine A,Mithieux G,Gautier-Stein A

更新日期：2012-03-01 00:00:00

abstract：:GABARAPL1 belongs to the small family of GABARAP proteins (including GABARAP, GABARAPL1 and GABARAPL2/GATE-16), one of the two subfamilies of the yeast Atg8 orthologue. GABARAPL1 is involved in the intracellular transport of receptors, via an interaction with tubulin and GABA(A) or kappa opioid receptors, and also par...

journal_title：Biochimie

authors： Seguin-Py S,Lucchi G,Croizier S,Chakrama FZ,Despouy G,Le Grand JN,Ducoroy P,Boireau W,Boyer-Guittaut M,Jouvenot M,Fraichard A,Delage-Mourroux R

更新日期：2012-03-01 00:00:00

abstract：:In the intestine, NF-κB is the main transcription factor involved in the anti-inflammatory effect of glutamine and we previously demonstrated that glutamine via its conversion to glutamate diminished the p65 protein content in Caco-2/TC7 cell nuclei without affecting the stimulating effect of IL-1β on NF-κB [21]. Howe...

journal_title：Biochimie

authors： Lesueur C,Bôle-Feysot C,Bekri S,Husson A,Lavoinne A,Brasse-Lagnel C

更新日期：2012-03-01 00:00:00